The study of species and biology of microflora isolated from 42 patients with chronic adnexitides has detected a wide spectrum of pathological agents among which both aerobic and anaerobic microorganisms were encountered. The isolated cultures were examined for ability to inactivate lysozyme, complement and lactoferrin. The experiments have established changes in biological properties of the microorganisms in response to use of mud and salty brine therapy. Clinical investigations demonstrate the ability of balneotherapy to restore normal flora, to reduce contamination with opportunistic microflora and suppress biological, including persistent, properties of microorganisms in females with chronic adnexitides. Positive effects of balneotherapy seem to base, primarily, on this ability.