Non-alcoholic fat liver disease (NAFLD) is an urgent problem of modern healthcare and ranks first among all liver diseases worldwide, assuming the nature of an epidemic. The pathogenesis of NAFLD is complex and insufficiently studied, and therefore pharmacological therapy is not always effective and causes negative side effects (or complications). Treatment with mineral waters (MW) is one of the most effective methods of treating diseases of the digestive system and metabolic disorders. Objective: to study the effect of sulphate MW on metabolic disturbances in rats with the experimental NAFLD model for the purpose of scientifically substantiating its use in the complex of treatment of patients. Morphological studies have established that the use of sulfate MW in healthy animals causes an increase in the functional activity of the liver and stomach, which indicates a pronounced biologic activity of this MW. In animals under the influence of sulphate MW was determined a significant reduction in hepatic steatosis according to the morphological study of biopsy specimens, restoration of the processes of bile formation and bile secretion, positive dynamics in the restoration of energy-dependent transmembrane ion transport. Clinical studies in the complex treatment of NAFLD with the use of sulphate MW established normalization of lipid metabolism, improvement of the functional state of the liver, decreased insulin resistance, recovery of adiponectin secretion.