The therapeutic effect of mineral water from spring 3 in BaileTusnad in experimental alcoholic liver disease in rats – an electron microscopic study

Authors: Dogaru G (1,2) , Stănescu I (1,2) , Bulboacă A (1,2) , Motricală M (3) , Rus V (4) , Crăciun C (5) , Toader S (6) , Rațiu IA (7)
(1) “Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca (2) Clinical Rehabilitation Hospital Cluj-Napoca (3) Tușnad Spa Complex-Băile Tușnad (4) University of Agricultural Sciences and Veterinary Medicine (5) Experimental Biology Department, Electron Microscopy Center, "Babeş-Bolyai" University (6) Experimental Medicine Center, “Iuliu Hatieganu” University of Medicine and Pharmacy Cluj-Napoca (7) University of Oradea, Faculty of Medicine, Medical Sciences Department
Source: Balneo Research Journal; Vol.9, No.3, September 2018 p: 211 –215
DOI: 10.12680/balneo.2018.183 Publication date: 2018 Sep E-Publication date: Not specified Availability: full text Copyright: Not specified
Language: English Countries: Romania Location: Băile Tușnad Correspondence address: Corresponding author: Vasile Rus, E-mail:


Article abstract

The aim of this study was to investigate by transmission electron microscopy the changes occurring in rat hepatocytes following administration of ethyl alcohol for 70 days, as well as to evaluate the effect of mineral water from spring 3 in Baile Tusnad balneoclimatic resort administered for 30 days after completion of ethyl alcohol administration. After 70 days of ethyl alcohol administration, lipid loading in hepatocytes and a reduction of the rough endoplasmic reticulum were observed. The smooth endoplasmic reticulum was found to be much more abundant and in some hepatocytes that contained high lipid amounts, mitochondria had rarefied matrix and cristae. At 30 days from completion of ethyl alcohol administration, the rats that drank tap water showed progressively decreasing lipid loading. Also, the smooth endoplasmic reticulum was less abundant, while the rough endoplasmic reticulum was more abundant than on day 70 of the experiment. The rats that drank mineral water from spring 3 compared to those that drank tap water over the last 30 days of the experiment had lower hepatocyte lipid loading and the rough endoplasmic reticulum was much more abundant. These ultrastructural differences in the degree of lipid loading and the ratio between the smooth and rough endoplasmic reticulum are due to the beneficial effect of mineral water from spring 3 in Baile Tusnad.

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